RNA Details
Disease Name
pulmonary hypertension
Tissue
lung
RNA Symbol
mir-27a
RNA ID
RNA Type
miRNA
Alteration Pattern
up regulation
Species
mus musculus
Detection Methods
Dual-luciferase reporter assay; qRT-PCR; etc.
Target
NA
Pathway
NA
PubMed ID
Title
Hypoxia mediates mutual repression between microRNA-27a and PPARY in the pulmonary vasculature
Year
2013
Function
"In hypoxia-induced PAH,miR-27a and ET-1 are increased, whereas PPARY is reduced. PPARY ligand rosiglitazone (RSG, 10 mg/kg/d) treatment could inhibit miR-27a expression.Overexpression of miR-27a or PPARY knockdown increased HPAEC proliferation and ET-1 expression and decreased PPARY levels. MiR-27a levels were upregulated in lungs from endothelial-targeted PPARY knockout (ePPARY KO) mice. Knockdown of either SP1 or EGR1 was sufficient to significantly attenuate miR-27a expression in HPAECs. Collectively, these studies provide novel evidence that miR-27a and PPARY mediate mutually repressive actions in hypoxic pulmonary vasculature and that targeting PPARY may represent a novel therapeutic approach in PH to attenuate proliferative mediators that stimulate proliferation of pulmonary vascular cells."